Last updated : May 19, 2025
A 5-panel test is commonly referred to as a “DOT 5-panel” and is required for DOT testing. It is also frequently used for non-DOT testing. In reality, a DOT 5-panel is more like a 14-panel test, as several categories have been expanded since the original panel was created. The amphetamine category also includes methamphetamine and ecstasy (MDMA and MDA) and the opioid category was expanded to include codeine, morphine, heroin, hydrocodone, hydromorphone, oxycodone and oxymorphone. These drugs were not as popular when the DOT began drug testing so the panel was updated to better reflect common drugs of abuse around the 2010 timeframe. The other drugs tested for in a DOT drug testing panel are: THC (marijuana), cocaine, PCP (Phencyclidine).
Currently, DOT drug testing is lab-based testing, where 45 milliliters of urine are sent to the lab. The sample undergoes a screening test and, if negative, meaning that there are no substances in the sample, the result is released as a negative result. If a substance is detected, the sample will then undergo confirmation testing using GC/MS (gas chromatography/mass spectrometry). This is highly sophisticated technology and is extremely reliable for analysis.
5-panel drug testing is reliable, comprehensive and widely accepted for most drug-free workplace programs, and required for all DOT drug testing.